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This highlights the functional consequences of earlier molecular lesions and reinforces the pivotal role of Increased proliferation and Decreased apoptosis as central KEs in lung cancer initiation within the AOPN framework

However, DMSO-free does not automatically mean safe because of the use of other cryoprotectants 55,56,57

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For instance, metformin can activate SIRT1 and regulate related pathways to mitigate LPS-induced SAKI and improve renal function of HFD-/STZ-induced diabetic rats (Xu et al., 2020