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The lipid residue in the LC-MS injection vial was redissolved with 300 L of isopropanol-methanol (V:V=1:1), vortexed for 30 s, and sonicated with ice water for 3 min, then the solution was transferred to a 1.5 mL centrifuge tube, and left to stand at -20C for 2 h

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This can trigger an endothelial-to-mesenchymal transition (94), where the loss of endothelial-specific markers and gaining of a mesenchymal or myofibroblastic phenotype contribute to fibrosis development due to the generation of activated myofibroblasts (95)