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One major mechanism of NMD escape involves cis-acting inhibitory elements within the mRNA, particularly in the 3UTR [38, 71]

Initial Treatment in Combination with Pembrolizumab and Platinum The efficacy of pemetrexed for injection in combination with pembrolizumab and platinum chemotherapy was investigated in Study KEYNOTE-189 (NCT02578680), a randomized, multicenter, double-blind, active-controlled trial conducted in patients with metastatic non-squamous NSCLC, regardless of PD-L1 tumor expression status, who had not previously received systemic therapy for metastatic disease and in whom there were no EGFR or ALK genomic tumor aberrations

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To this end, a 96-well Nunc Maxisorp plate (Thermo Fisher Scientific) was coated with 5 g/mL human CEA-Fc chimera (Genscript, Piscataway, NJ) or HER2 extracellular domain (EMP Genetech, Ingolstadt, Germany) in PBS (4 mM KH 2 PO 4 , 16 mM Na 2 HPO 4 , 115 mM NaCl, pH 7.4) at 4 C overnight and blocked with 3 % (w/v) bovine serum albumin (BSA) in PBS/T (PBS supplemented with 0.1 % (v/v) Tween-20) for 2 h at room temperature

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