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LT-HSCs obtained from the Gpx4 f/f mice or Gpx4 / mice (CD45.2) together with BM cells obtained from competitor mice (CD45.1) were transplanted into recipient mice (CD45.1.2), whose hematopoietic system was destroyed by a lethal dose of X-ray irradiation (Fig

On the day of the assay, the culture medium was replaced with Seahorse XF RPMI assay medium (103576-100, Agilent) supplemented with 1 mM pyruvate, 2 mM glutamine, and 10 mM glucose

Most tumor cells can produce IL-6, which promotes increased PD-L1 expression in neutrophils by activating JAK/STAT3 signal transduction, assisting the tumor in escaping immune surveillance