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RNA extraction from homogenates was performed following the Tri-Reagent protocol, with the addition of an isopropanol washing step before ethanol precipitation

Vemuri et al

This was described by the original authors as "very surprising" and represented a categorically different selectivity profile from all previously characterised GHRP receptor agonists

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In mice treated with gradually raised doses of lipopolysaccharide, significant increases in the activity of the signaling pathways tested, heat-shock protein and TLR4 expression, lymphocyte apoptosis, and plasma proinflammatory cytokine levels were noted