( a ) RAW 264.7 macrophages were infected with 1:1 mixtures of wild-type Salmonella strain (STM WT ) or sirM (STM sirM ) and streptomycin-resistant wild-type Salmonella (STM-Str)
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Sequential centrifugation steps are performed at incrementally increased speeds, for example, 300-3,000 g for cellular debris, 10,000 g for organelles, and 100,000 g for PDEVs, followed by resuspension of the pelleted vesicles in a biocompatible buffer such as PBS to minimize contaminants [78,79,80,81,82]
Superoxide dismutase expression attenuates cigarette smoke- or elastase-generated emphysema in mice