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the use of pro-GSH molecules (Fraternale et al., 2006) and/or glutathione precursors like N-acetyl cysteine (NAC), glutamine, cysteine (cystine) and glycine, as well as nuclear factor erythroid 2 p45related factor 2 (Nrf2) inducers like sulforaphane can enhance glutathione production and increase nuclear Nrf2 translocation and antioxidant response element (ARE) transcription (Komaravelli and Casola, 2014

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It should be noted that the aforementioned report lack data substantiating GCLC deletion or glutathione synthesis inhibition

It has been proposed that epithelial and mesenchymal cells survival relies on the signaling provided by integrins attached to a rigid surface and that culturing them in 3D gels leads to cell death by preventing attachment to a rigid surface [26]