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Methods: Female wild-type (WT) and Has1,Has3 double knockout ( Has1,3 dko) mice were aged to 7 weeks (young) or 17 months (old) and fed normal mouse chow (5% calories from fat)

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Legumain, also known as asparaginyl endopeptidase (AEP), is associated with the caspase family due to its approximately 15% sequence homology with caspases and its strict specificity at the P1 position [10, 11]

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