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Description

Purified antibodies were quantified by OD280 using an extinction coefficient calculated from the primary amino acid sequence

Column chromatography on silica gel was carried out with Merck Kieselgel 60 silica (230400 mesh)

Hormonal fluctuations, particularly during pregnancy or when taking oral contraceptives, can trigger melasma, a type of hyperpigmentation characterized by symmetrical brown patches typically appearing on the face