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In human somatic cells, telomerase is normally silenced after embryonic development, meaning telomeres shorten with each cell division until reaching a critical length that triggers replicative senescence or apoptosis

Additionally, individuals who work in jobs that require prolonged standing, such as teachers or retail workers, may experience similar discomfort

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Gradient elution starts from liquid A accounting for 100% of the mixture, during which the proportion of liquid B in the mixture gradually increases until it accounts for 100%, the flow rate is 10ml/min, and the detection wavelength is 215nm