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Stain-free gels were then wet-transferred onto polyvinylidene difluoride (PVDF, Millipore) membranes at 300 mV constantly for 1 h, and equal transfer was routinely confirmed by Ponceau S staining

It is a powerful antioxidant produced in the body and found in every cell, primarily in the liver

Shatillo A, Koroleva K, Giniatullina R, Naumenko N, Slastnikova AA, Aliev RR, Bart G, Atalay M et al (2013) Cortical spreading depression induces oxidative stress in the trigeminal nociceptive system

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