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In this protocol, 10 mg of rNS1 that had undergone denaturation with urea was diluted to a concentration of 100 mg/ml before the refolding step

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As a gasotransmitter, H 2 S is known to exert anti-inflammatory, antioxidant, and vasodilatory effects, which are particularly relevant in the pathogenesis of high pulmonary blood flow-induced PH, a condition characterized by vascular remodeling, immune cell infiltration, and elevated pulmonary pressures

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