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In 5000 ml of ethanol/dioxane (1:1), the acylated compound was dissolved, Pd/C was added, and the mixture was stirred at room temperature under hydrogen at atmospheric pressure for about 2 h

Modeling threonine in the same orientation as serine onto the active site would induce clashes between the threonine methyl group and the Cys residues, forcing threonine into a less productive conformation for activation (Bilokapic et al

Superoxide Dismutase (SOD), otherwise known as the Youth Molecule, is the only antioxidant agent that is able to regulate the production of superoxide anion radicals