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Kanbar et al., 2023)

282 However, they have encountered several challenges

Komm BS, Kharode YP, Bodine PVN, Harris HA, Miller CP, Lyttle CR

Isolated mitochondria were resuspended in mitochondrial assay solution (70 mM sucrose, 220 mM mannitol, 10 mM KH 2 PO 4 , 2.5 mM MgCl 2 , 2.5 mM NaCl, 2 mM HEPES, 1 mM EGTA, 4 mM ADP, and 0.2% (w/v) fatty acid-free BSA, pH 7)