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Therefore, when Annexin V was used in combination with PI, PI was excluded from living cells (Annexin V-/PI-) and early apoptotic cells (Annexin V+/PI-), while late apoptotic and necrotic cells stained double-positively (Annexin V+/PI+) with both FITC and PI

186 Guidelines include 186 : Eat the majority of the days calories in the daytime (rather than at night)

When needed, statistically significant effects were followed up using pairwise comparisons with the Tukey adjustment to account for multiple comparisons