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The study also found that older patients had a greater reduction in pain after injection

Enzyme expression was induced by the addition of 100 M isopropyl -d-1-thiogalactopyranoside, the cultures were incubated for another 3.5 h at 30 C, 140 rpm using a 5 cm shaking diameter, and the final OD 600 (optical density at 600 nm) was determined using a CO8000 cell density meter (WPA biowave)