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Some patients likely saw dramatic improvement while others saw less

One interesting study used a network pharmacology approach to show that the TCM herb pair Gui ZhiShao Yao (Cinnamomum cassia & Paeonia lactiflora) may treat chronic pain with cooccurring anxiety and depression through 15 active compounds acting on 130 targets, with nine core proteins (including Akt1, IL6, TNF, PTGS2, and PPAR) and 11 key inflammatory and stress-related pathways (such as AGERAGE, IL17, and TNF signaling), suggesting a multi-target, systems-level mechanism that still needs confirmation in experimental models

Given the poor bioavailability of oral NAD+ and its precursor vitamins through supplements (and why most wellness supplements have limited benefit), IV or subcutaneous routes are necessary to achieve the serum concentrations observed in the published research

Timing considerations Morning and evening applications are common for twice-daily protocols

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