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Methods 14 , 587589 (2017)

The standard route in research community use and in most published guides

It indicated that the knockdown of MKL-1 expression regulated the occurrence of ferroptosis in ferroptosis stress model by affecting the synthesis of GSH

The first consists of directly boiling the seeds in water for 1 h, and the second involves a pre-soaking step for one night followed by boiling in water for 25 min