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After the immersion with the blocking buffer (10 mM Tris-HCl, pH 7.4, containing 0.5 M NaCl, 5% skim milk, and 0.5% Tween-20), the membrane was incubated with anti-APAP pAbs, anti-CYP1A2 pAbs, anti-CYP2E1 pAbs, anti-CYP3A pAbs, or anti- -actin pAbs at 4C overnight

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Kynurenine acts as a signaling molecule to attenuate pulmonary fibrosis by enhancing the AHR-PTEN axis