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For in vivo experiments, one aliquot was thawed and cultured in Schneiders medium for maximally 4 to 8 passages

Mice were fasted for 16 to 18 h before receiving APAP by intraperitoneal injection (Hu et al

doi: 10.1016/j.autrev.2009.02.020

Yet most published protocols overlook the temporal coordination required to capture both compounds at peak efficacy

Rather than targeting a single mechanism, it simultaneously promotes blood vessel formation (angiogenesis), enhances growth factor signaling, increases nitric oxide for better tissue oxygenation, and suppresses inflammatory markers