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Introduction Enzymes are a class of biological macromolecules with catalytic activity and specific spatial conformation in organisms, and they are widely used in chemical industry, medicine, food, and energy ( Candida rugosa lipase (CRL) is a lipase with important application value, which has high activity and specificity and can be widely used in chemical, cosmetic, food, and other applications ( Immobilization can improve the operational stabilization and activity of enzymes and sometimes can even modulate the selectivity of enzymes by immobilization on functional supports ( 3 O 4 and natural organic polymers with the characteristics of excellent biocompatibility, high mechanical stability, and good recyclability have received more and more attention and obtained good applications in enzyme immobilization ( 3 O 4 ) that consisted of magnetic graphene oxidechitosan (GO-CS) and magnetite nanoparticles (Fe 3 O 4 ) and that was activated with glutaraldehyde (GA) to immobilize CRL