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doi: 10.3389/fpls.2020.00359 119 YangJ

Metabolites were extracted using ultrasonic treatment in an ice bath for 30 min and then centrifuged for 10 min at 12,000 rpm

Axon-soma communication in neuronal injury

Following incubation, oocytes and blastocysts were washed three times in DPBS (1x) containing PVP (0.1%) and immediately imaged using an inverted fluorescence microscope (IX73, Olympus, Tokyo, Japan)