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Cells were washed twice with PBS and then 0.5 ml NP-40 lysis buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.5% (w/v) NaDOC, complemented with 0.5% (v/v) NP-40 (Sigma-Aldrich, Steinheim, Germany) and 1x Roche Protease Inhibitor w/o EDTA (Roche Diagnostics, Penzberg, Germany) were added to the cells

The neurons were treated with gadolinium chloride for different time periods

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[72] Isolation of lactoferrin from milk