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HBOT is a medical treatment which introduces 100% oxygen to the patient in a painless and relaxing atmosphere

The reaction yields a colorimetric signal where samples with reduced amounts of SOD emit a lesser signal that can be measured via colorimetry ( Catalase CAT is quantified by adding hydrogen peroxide (H 2 O 2 ) to the sample and analyzing the rate of decomposition of CAT, which is proportional to a reduction in the absorbance reading generated by the instrument ( Reduced Glutathione GSH can be determined by adding 5,5-dithiobis-2-nitrobenzoic acid (Ellman's reagent) to the sample

Moreover, this study also indicated that GLS inhibition significantly impaired the production of antioxidant glutathione in types of AML cell lines, leading to increased mitochondrial ROS (mitoROS) and cell apoptosis [11, 17]

Also, CML levels correlated with severity of disease [99]

Clinical Science 2009, 116 (5-6), 403-413