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Western blot analysis revealed elevated SHP-1 expression and reduced phosphorylated STAT3 (p-STAT3) expression in glioblastoma cells treated with AC compared to controls

Each Cy5-labelled DNA was added to hybridization buffer (20 l DIG Easy Hybridization Buffer, 1 l 10 mg/ml Yeast tRNA and 1 l 10 mg/ml Salmon Sperm DNA) and hybridized for 18 hours at 50 C followed by washing 3 1 SSC/0.1% SDS at 42 C and final wash in 0.1 SSC at RT

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These observations suggest a role for epigenetic modifications in PD

[DOI] [PMC free article] [PubMed] [Google Scholar] 4.Harper JM, Leathers CW, Austad SN