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One meta-analysis concluded that there was no significant relationship between tea consumption and the risk of GERD

Tissue culture infectious dose at 50% (TCID 50 ) were then determined by serial dilution on near confluent Madin-Darby canine kidney (MDCK) cells (purchased from American Type Culture Collection (ATCC CCL-34)) in the presence of 1 mg/ml trypsin (Sigma Aldrich), verified with 50% egg infectious doses (EID 50 ) and calculated according to the Reed and Muench method [120]