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The distance from the blood supply can be up to 8 mm or more ( Under normal physiological conditions, the center of the nucleus pulposus primarily relies on anaerobic glycolysis for metabolism, forming a nutrient gradient from the periphery to the center, characterized by a microenvironment with low glucose (approximately 1 mmol/L) and high lactate (approximately 5 mmol/L) (Urban, 2002)

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After the subcutaneous tissues are inflated, a varying number of 5 mm skin incisions (depending on the need) are made near the previously marked areas

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