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SDSpolyacrylamide gel electrophoresis (PAGE) and Western blotting (WB) Huh7-Lunet neoJFH1 or transfected Huh7-Lunet T7 cells seeded in six-well plates were lysed with 100 L lysis buffer (50 mM TrisHCl pH 7.4, 150 mM NaCl, 1% Triton X-100) supplemented with protease inhibitor (Roche)