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Except for tumor cells, TME consists of stromal cells, such as tumor-associated fibroblasts (CAFs) and lymphocytes, including T cells, unconventional T cells, B cells, natural killer cells (NK), neutrophils, tumor-associated macrophages (TAMs), and myeloid-derived suppressor cells (MDSCs) in HCC

The 24-h UTP, serum scr and serum BUN concentrations of mice were detected with the specified detection kit

Int J Mol Sci (2019) 20(15):3775

6.1 Regulation of reproductive-related hormones The HPO axis is a highly coordinated neuroendocrine system integrating the hypothalamus, pituitary gland, and ovaries to regulate reproductive cyclicity

Cell culture and transfection/electroporation 293 T human embryonic kidney cells were cultured in Dulbeccos modified Eagles medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, 100 units/mL of penicillin, and 100 g/mL of streptomycin and then transfected with expression vectors via the calcium phosphate-DNA precipitation method 33