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The tumor tissues were ground and lysed in radioimmunoprecipitation assay lysis buffer (#P0013B, Beyotime Institute of Biotechnology, Haimen, China) containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 1% sodium deoxycholate, 1 g/ml leupeptin and 0.1% SDS on ice, and then centrifuged at 12,000 g, 4C for 20 min to obtain the total protein supernatants

doi: 10.1016/j.hermed.2014.11.001

Last day of work was on Friday, and that went well

Carter BG, Cheng N, Kapoor R, Meletharayil GH, Drake MA

However, zymography overruled interferences observed in the spectrophotometric method where inhibition of the enzyme can be classified based on color band formation corresponding to the activity of an enzyme