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To test whether the protective effect of cysteine in ES2 and OVCAR3 cells is concentration-dependent, cells (5 10 5 cells/well) were seeded in 6-well plates and cultured in control condition and exposed to 0.1 mM, 0.2 mM, 0.4 mM, 0.8 mM and 1.0 mM L-cysteine and/or hypoxia induced with 0.1 mM cobalt chloride

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[116] further elaborate on how gut metabolites can directly regulate the functional states of microglia, or indirectly regulate them via the GBA, and their role in the pathogenesis of neurodegenerative diseases and NDDs