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After removal of the supernatant, the aggregates were resuspended in 3.5 ml/million cells of differentiation medium comprised of RPMI 1640 (Life Technologies) with B-27 either with or without insulin (RPMI/B-27 +/-) (Life Technologies) depending on the cell line and 3 or 4 M CHIR99021 (BOC Sciences)

Similar content being viewed by others Introduction A successful treatment of a sentinel case of Bells Palsy was reported in 2020, utilizing adipose-derived stem cells [1]