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To this LSB-2, different NaCl concentrations (75, 150 and 300 mM), IGEPAL CA-630 (1%) and increased buffer volume (1250, 1875 and 3750 L, respectively giving rise to ratios of ~ 1.6 10 12 , ~ 1.06 10 12 , ~ 0.53 10 12 cells/ml of buffer) for a 250 ml culture pellet was used along with BugBuster Protein Extraction Reagent and Sonication conditions were tested

Nature (2006) 444(7122):10223

PLoS One 9 (5), e96820

Relationship between serum concentrations of persistent organic pollutants and the prevalence of metabolic syndrome among non-diabetic adults: results from the National Health and Nutrition Examination Survey 1999-2002