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Real-time quantitative PCR (RT-qPCR) The total RNA was extracted from leukocytes using the QIAamp RNA Blood Kit (Qiagen, Hilden, Germany), and cDNA was produced with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher, Waltham, MA) as previously described 18

Shots ensure direct absorption, ideal for those with deficiencies

Acknowledgements We acknowledge the following team members, collaborators and other staff who have contributed to this study