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(Figure 3A,B) Consider in vivo GSH concentration of 110 mM, second-order reaction rate k GSH around 0.048 0.48 M 1 min 1 translates to a half-life of 24 h

doi: 10.1016/j.isci.2023.107487 51 WangDWangXTanBWenXYeSWuYet al

In the oxidative half of the mechanism, Cys63 nucleophilically attacks the nearest sulfide unit in the glutathione disulfide (GSSG) molecule, which creates a mixed disulfide bond (GS-Cys58) and a GS- anion