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It's a sophisticated method of removing water from the peptide by freezing it and then reducing the surrounding pressure to allow the frozen water to sublimateturning directly from solid ice to vapor

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a method described herein e.g., shuffled to recombine combinatorially the amino acid sequence of the selected polypeptides, peptide(s) or predetermined portions thereof

Pre-oligodendrocytes, which are more vulnerable than immature neurons or mature oligodendrocytes, are most affected (Haynes et al., 2003)