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Analysis of the biochemical biomarkers Preparation of the samples Samples were homogenized at a ratio of 1/4 (weight/volume) with a homogenization buffer and then centrifuged at 20,000 for 20 min at 4 C, according to Bainy et al

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coli isolates where 0.312 mg/ml was the mean concentration for its anti-biofilm effect, similar results were reported by Shivaprasad et al.who detect that vitamin C-treated bacterial culture showed a 5.8 folds reduction in EPS production in comparison to control without vitamin C [4, 23]

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The realm of non-coding RNA (ncRNA) regulation encompasses microRNAs (miRNAs), which inhibit gene expression by targeting the 3-untranslated region (3-UTR) of mRNAs, as well as long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) that regulate gene expression by competitively binding to miRNAs