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Description

2008 May 28 (5):886-91

It's a complementary mechanism, not a standalone solution

2010;91(5):1461S-1467S

The supernatant from the hypotonic solution was combined with the supernatant from the hypertonic solution, centrifuged to remove debris, and dialyzed against 20LmM Tris, pH 7.5 and 150LmM NaCl (Tris-buffered saline, TBS) overnight at 4 C