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Once completed, the cells were washed with media one time, then 2 mls of vehicle or conditioned media were added to the wells

It seems possible that oxidation of these thiols to disulfides, under a mechanism of oxidative stress, would increase the affinity of SARS-CoV and SARS-CoV-2 S proteins for the ACE2 receptor, and therefore, increase the severity of COVID-19 infection [30, 60]

Limitation of the study This study had several limitations

Amyotrophic lateral sclerosis ALS is a neurodegenerative disease where the degeneration of motor neurons dominates the clinical presentation and the course of the disease, which is fatal within a few years from onset