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3.4 Inhibition of intracellular synthesis of reduced glutathione does not impair its extracellular action In the next experiment, buthionine sulfoximine (BSO, 10mM), the inhibitor of -glutamylcysteine synthetase was added during 24 hours to A549 cell culture (Figure 6)

CrossRef Endothelial GNAQ p.R183Q mutation confers hemoporfin-mediated photodynamic therapy resistance and drives pathological angiogenesis via the angiopoietin-2/TIE2/PI3K/AKT pathway Lu Liu, Linmei Wang, Jiaxin Luo, Jiayun Yu, Liang Xie, Yang Liu, Hong Xu, Fan Hu, Hanmin Liu Frontiers in Cell and Developmental Biology.2025;[Epub] CrossRef Gold nanoparticle-mediated photothermal therapy: Expanding the frontiers of cancer treatment and theragnostics Mariana Neves Amaral, Pradeep Kumar, Pedro Fasca, Hugo Alexandre Ferreira, Joo M.P

In addition to the cell death modalities mentioned in this review, mitochondria act as direct drivers in multiple contexts: in mitochondrial permeability transition-driven necrosis, the opening of the mitochondrial permeability transition pore leads to loss of membrane potential and energetic collapse, serving as a direct trigger for cell death (Tsujimoto et al., 2006)