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This fragment was digested with EcoRI (NEB) and ligated into pGex-6p1 (GE Healthcare) prepared by digestion with BamHI and blunting with Mung Bean Nuclease (NEB) to generate pJB-GST-HTS

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This approach differs from World Anti-Doping Agencys, which stopped restricting caffeine use in 2004, in part because of caffeines widespread social acceptance and because of its questionable benefits at high levels

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