Ten-m thick sections were cut, mounted on glass slides, air-dried at RT, and kept at -20 C
These investigations remain confined strictly to laboratory research contexts and do not represent approved therapeutic use
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Together, these results support the view that neutrophils are important targets for the diagnosis and therapy of RA
However, lower concentrations (typically ~ 10-50 mM) of imidazole are used in the binding and wash buffers to prevent proteins with a few opportune histidine residues from binding to the nickel column (Hemdan et al., 1989
