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2.2 Metformin alleviates endoplasmic reticulum (ER) stress ER stress refers to a homeostatic imbalance within the ER caused by external stimulus or changes in the intracellular environment, which further leads to the accumulation of unfolded or misfolded proteins in the ER

Only metabolites present in 50% of the samples were measured, and metabolites whose coefficient of variation value was over 30% in pooled QC samples were removed from analysis

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Several factors, including cytokines, proteases, growth factors, gut microbiota, and dietary components are known to regulate intestinal TJs opening ( Intestinal epithelial cells and mononuclear phagocytes sense bacteria or their products mainly through pattern recognition receptors such as TLRs (166)

The metabolic profile is very similar to that in column 3 except that now the intracellular SAH concentration does not decline but instead increases by 24% in line with the clinical data