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Diagnosis requires full blood count showing macrocytic anaemia, serum B12 measurement below 148 pmol/L, and intrinsic factor antibodies to confirm pernicious anaemia

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" But your experience does not generalize - many people with PA experience returning symptoms although their serum B12 is normal or high

Undigested cross walls and debris were removed by centrifugation (10 min at 50,000 g ) and crude nozzle preparations were collected in the ultracentrifuge (1 h at 366,000 g ) before being further purified using a CsCl density gradient (0.3 g/ml)