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6,11 Differential diagnosis should include gastrointestinal (GI) abnormalities, central nervous system disorders, kidney stones or gallstones, metabolic-endocrine disorders, appendicitis, and inflammatory bowel disease

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Therefore, the best metabolomic practices are essential to overcome this initial phase, for instance, conducting studies that involve large numbers of subjects, assessment of environmental influences on breath samples, implementation of reproducible and transparent data preprocessing workflows and performing a robust data analysis, among others 29,33