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This step is followed by treatment with an azide-tagged fluorophore, after which cellular fluorescence is measured using a flow cytometer or a plate reader in 96- or 384-well plates

Interestingly, on undergoing a LOsG/ROS stress, -cells started to re-express fetal nuclear Neurog3 and to dramatically relocate pancreas/duodenum homeobox protein 1 (Pdx1) from the cytoplasm to the nucleus (Fig