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We minimised the effect of the high background due to Rayleigh scattering by subtracting a linear baseline (black line) from the raw UV-Vis data (green line) (Fig

10.1016/j.nbd.2015.07.003 86 RowleyS.PatelM

One of the enzymes in this pathway, -butyrobetaine hydroxylase, is absent from cardiac and skeletal muscle but highly expressed in human liver, testes, and kidney [4]

We then appended poly-d-alanine tails of different lengths

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