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As a major antioxidant, glutathione interacts with other antioxidants in the body, such as alpha-lipoic acid, vitamin C, and vitamin E, and they mutually enhance each others work

Subsequently, daily oral gavage of metformin (200 mg/kg/day) commenced on the second day post-surgery and continued until euthanasia

16S rRNA gene sequencing and metagenomics are some of the high-throughput sequencing technologies with the ability to identify patient-specific dysbiosis signatures

This first step is also the most critical rate-limiting step

Size exclusion chromatography Gel filtration experiments were carried out on a Superdex 200 10/300 GL column (manufacturer's exclusion limit 600 kDa for proteins) on an KTA-FPLC (GE Health Care Biosciences)