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intro Tirzepatide is a dual GIP and GLP-1 receptor agonist manufactured by Eli Lilly, available under two brand names: Zepbound (FDA-approved for chronic weight management and obstructive sleep apnea) and Mounjaro (FDA-approved for type 2 diabetes)

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RNA extraction and RT-qPCR Total RNA was extracted from frozen specimens and kidney cancer cell lines using TRIzol reagent (9108, Takara, Japan) and cDNA was generated using Evo M-MLV Reverse Transcription Premix Kit (AG11728, Accurate Biology, China)

292 This approach enables selective oriented AuNP dimerization upon target binding, greatly improving long-term stability and broadening the detection range over two orders of magnitude compared to conventional sensors

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