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Transgenic zebrafish seedlings at 3 days post-fertilization (3 dpf) were selected and transferred into 24-well plates containing 5 seedlings/well

Protocols for De Quervains typically involve subcutaneous injection approximately 2 inches proximal to the radial styloid process, the bony bump on the thumb side of your wrist

Side effects are generally mild and rare but can include temporary flushing, a metallic taste, or minor injection site discomfort

These seemingly minor issues can have significant implications for your research

The interpretation of these transcriptomic findings has been a continuing area of discussion, with some researchers viewing the broad effects as evidence of a regulatory function and others viewing them with caution given the difficulty of distinguishing direct effects from secondary consequences in such broad gene-expression landscapes